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Research Technologist Sr

Boston, MA

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Description We are recruiting an experienced Technologist to also serve as the Laboratory Manager. The Spriggs Lab is a translational laboratory focused on the Biology of Ovarian Cancer, particularly the MUC16/CA125 glycoprotein and its effects on the growth, invasion and metastatic behavior of Ovarian cancer.The laboratory will eventually reach a size of 5-8 people including students, technical staff and post-doctoral fellows.The laboratory is a highly collaborative team which participates in the conduct and publication of studies with diagnostic / therapeutic implications for the treatment of human malignancy.We are looking for an experienced and ambitious individual to assist in all experimental aspects of the laboratory's work, including animal studies, as well as manage daily operations.Opportunities for publication and progress toward independence will be provided. SPECIFIC DUTIES Operations -Maintain equipment, inventory and protocols, purchasing, and related inquiries. -Coordinate lab schedules and resolve technical problems.u00a0 -Provides instruction to new staff in various laboratory techniques, including common molecular biology skills such as cell culture ELISA, FACS vector design and construction, PCR, DNA cloning protein analysis and protein production / purification and storage. -Supervise the activity of laboratory technicians and assist post-doctoral fellows with new techniques and protocols. -Develop and implement laboratory(s) policies and procedures in conjunction with the PI.u00a0Writes and updates laboratory protocols and safety guidelines.u00a0Coordinate the production of MAPS, Standard Operating Procedures. -Frequently interacts with other labs and coordinates the program activities with responsibility for results in terms of costs, methods, and employees. -Monitor compliance with good animal laboratory practice in accordance with IACUC requirements .u00a0 -Advises on technical procedures, techniques and equipment, and maintain conformance with specific operational standards. -Installs and maintains laboratory equipment in compliance with federal regulations.u00a0Develops and implements quality assurance programs relating to calibration and maintenance of equipment. -Oversees chemical safety and biosafety training and records for the lab.u00a0 -Monitors all lab personnel for adherence to NRC guidelines and MGH safety standards. -Assists in the MTA process, following through on material requested in or out of the laboratory. -Assist the PI and Grant Manager with expense monitoring. Bench -Conducts experiments and performs routine and technically advanced assays as needed. -Performs protein purification for projects and laboratory. Completes all stages of preps from growth to purification and characterization of material.u00a0 -Participates in the development and investigation of new procedures, assays, and/or instrumentation.u00a0 -Performs specialized experiments with a high level of independence.u00a0 -Contributes to improvements in experimental protocols and techniques. Creates new protocols as needed. -Maintain records on cell lines, vectors, DNA sequences and antibodies. -Participates in the preparation of data and text for presentation and publication Qualifications B.S or B.A required in Biochemistry, Molecular Biology, or related field. Immunology training a plus. Advanced degree preferred. A minimum of 5 years of progressively more responsible experience in a research field required. Must have proficiency in laboratory methods of molecular biology or immunology, and experience with antibody characterization and development preferred.u00a0Animal skills for treatment, tumor measurement and imaging. Aptitude and technical problem solving;u00a0attention to detail;u00a0u2022excellent oral and written skills; organizational skills to formulate and complete time tables; excellent work habits and adaptability changing work environment;proficient in Office and database software.

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